Elisa Kit - Professional Manufacturer directly supply.

• ELISA kits are ready-to-use immunoassay kits that contain all reagents required to perform the 
• ELISA (Enzyme-Linked Immunosorbent Assay). ... 
• Antibodies linked with enzymes are used to quantify the results. 
• Various formats (see below) exist and the antibody antigen interaction principle is different for each format.
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  How to choose the Elisa kit? 10 tips to choose right Elisa kit. 
  
  

  1. The species studied

  If the sample is from a classical model such as human, mouse, and rat, it is relatively easy to find a validated ELISA kit. 

  But if the sample is from a non-classical model such as monkey, there are limited numbers of commercial ELISA kits available. In this case, you may have to choose a kit validated on species that shows homology with the species of your sample.

  
  

  2. The analyte detected

  You should clearly understand what kind of analyte (usually protein) to detect. A BY ELISA is generally suitable for detecting large proteins with multiple epitopes such as a cytokine. A competitive ELISA is appropriate for detecting small molecules like hapten.

  
  

  Most commercial ELISA kits are validated on serum/plasma and culture supernatants. It is important to read the product instructions in detail to ensure that the kit is compatible with your sample. For example, the way that plasma samples are collected (heparin or EDTA) can affect which ELISA kit should be chosen. Besides, other factors such as hemolysis and the presence of lipids in the sample can interfere with assay performance. So take these factors into consideration before choosing an ELISA kit. Purchasing a trial size Elisa kit to do a pre-test with your samples is recommended to verify whether the kit is suitable and sensitive to the sample.

  
  

  3. Purpose of the analysis

  ELISA is a tool that can be used for both qualitative and quantitative analyses. Qualitative ELISA provides a simple positive or negative result for a sample, while quantitative ELISA reflects the concentration of the analyte in a sample via a standard curve. Do you want to quantify the analyte in your sample or only to detect the presence/absence of it  Based on the purpose of the analysis, you can choose between qualitative ELISA and quantitative ELISA.

  
  

  4. Type of antibodies

  You can consult the ELISA kit suppliers what types of antibodies are used in the kit: monoclonal or polyclonal antibody  In sandwich ELISA, it is sometimes helpful to use a polyclonal antibody for capture and a monoclonal antibody for detection.

  
  

  5. Requirement of sensitivity

  If you don't have a clue about the concentration of the analyte in your sample, ELISA kits with a broad detection range are a better choice. If the concentration of the analyte in the sample is very low, ELISA kits with high sensitivity are recommended. If the concentration of the analyte is too high, dilution of samples may be made to adapt to the detection range of the ELISA kit.

  
  

  6. Sample size

  ELISA kits usually require from 100ul down to 10ul sample. If the amount of your sample is very small or your sample is very precious, you'd better choose ELISA kits that require less amount of sample.

  
  

  7. Recovery and linearity data

  Recovery and linearity experiments are used to assess the performance of ELISA kits. Recovery helps determine whether analyte detection is affected by differences in sample matrices. High recovery is better. The linearity of dilution determines the extent to which the dose-response of the analyte is linear in a particular diluent. Ideally, the concentration of the samples should be similar for all dilutions.

  Most suppliers provide recovery and linearity data on product specifications. Besides, other important parameters such as sensitivity and dynamic range are also provided. ELISA kits of different manufacturers may have different parameter data. You can carefully compare these parameter data, particularly recovery and linearity data, to choose a right ELISA kit.

  
  

  8. Detection system

  There are several different detection systems in ELISA, including colorimetric, fluorescent, and luminescent methods. All ELISA involves the immobilization of the analyte to a surface as well as the use of an enzyme label and a matching substrate. Choosing an appropriate enzyme and a matching substrate is important. Moreover, enzyme-substrate reaction conditions, the microplate, and the detection device should be properly chosen.

  
  

  9. The experimental protocol

  ELISA kits with simple protocols, convenient operation, and short experiment time will make it easier for you to do an ELISA test.

  
  

  10. Reference

  ELISA kits that have been used by other researchers and reported in the literature are typically more trustworthy. Additionally, manufacturers and products that have received certification are generally more reliable.

  

  
  
  

   

   

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